Construction of an Optimized Helper Plasmid Containing VPX for Lentiviral-Mediated Transduction of Dendritic Cells
نویسندگان
چکیده
منابع مشابه
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Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs.
Understanding how to achieve efficient transduction of hematopoietic stem and progenitor cells (HSPCs), while preserving their long-term ability to self-reproduce, is key for applying lentiviral-based gene engineering methods. SAMHD1 is an HIV-1 restriction factor in myeloid and resting CD4+ T cells that interferes with reverse transcription by decreasing the nucleotide pools or by its RNase ac...
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ژورنال
عنوان ژورنال: Biomedical and Pharmacology Journal
سال: 2017
ISSN: 0974-6242,2456-2610
DOI: 10.13005/bpj/1087